Abstract
A cDNA encoding one of two major allotypic forms of bovine pancreatic preprocarboxypeptidase A (preproCPA) has been cloned, and its entire nucleotide (nt) sequence determined. The cloned cDNA contains a 26-nt 5'-noncoding region, a 1260-nt open reading frame and a 51-nt 3'-noncoding region. The amino acid (aa) sequence deduced from the gene sequence contains Ile179, Ala228 and Val305 at allotypic aa residues. The procarboxypeptidase A (proCPA) was produced in yeast and secreted into the medium. Upon treatment with trypsin, proCPA generated an active enzyme with the same size as the mature carboxypeptidase A (CPA), as analyzed by use of anti-CPA polyclonal antibody.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigenic Variation / genetics
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Base Sequence
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Carboxypeptidases / biosynthesis
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Carboxypeptidases / genetics*
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Carboxypeptidases / metabolism
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Cattle
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Cloning, Molecular
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DNA, Complementary / genetics
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Enzyme Activation
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Enzyme Precursors / biosynthesis
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Enzyme Precursors / genetics*
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Enzyme Precursors / metabolism
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Genes / genetics*
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Molecular Sequence Data
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Pancreas / enzymology*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Sequence Analysis, DNA
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Trypsin
Substances
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DNA, Complementary
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Enzyme Precursors
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Recombinant Fusion Proteins
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Carboxypeptidases
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preprocarboxypeptidase A
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Trypsin