Cloning, sequencing and expression of the gene encoding a major allotypic preprocarboxypeptidase A from bovine pancreas

Gene. 1995 Nov 20;165(2):333-4. doi: 10.1016/0378-1119(95)00454-e.

Abstract

A cDNA encoding one of two major allotypic forms of bovine pancreatic preprocarboxypeptidase A (preproCPA) has been cloned, and its entire nucleotide (nt) sequence determined. The cloned cDNA contains a 26-nt 5'-noncoding region, a 1260-nt open reading frame and a 51-nt 3'-noncoding region. The amino acid (aa) sequence deduced from the gene sequence contains Ile179, Ala228 and Val305 at allotypic aa residues. The procarboxypeptidase A (proCPA) was produced in yeast and secreted into the medium. Upon treatment with trypsin, proCPA generated an active enzyme with the same size as the mature carboxypeptidase A (CPA), as analyzed by use of anti-CPA polyclonal antibody.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigenic Variation / genetics
  • Base Sequence
  • Carboxypeptidases / biosynthesis
  • Carboxypeptidases / genetics*
  • Carboxypeptidases / metabolism
  • Cattle
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Enzyme Activation
  • Enzyme Precursors / biosynthesis
  • Enzyme Precursors / genetics*
  • Enzyme Precursors / metabolism
  • Genes / genetics*
  • Molecular Sequence Data
  • Pancreas / enzymology*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Trypsin

Substances

  • DNA, Complementary
  • Enzyme Precursors
  • Recombinant Fusion Proteins
  • Carboxypeptidases
  • preprocarboxypeptidase A
  • Trypsin

Associated data

  • GENBANK/Z33906