Abstract
SRY (sex-determining region of the Y chromosome) has recently been identified as a key regulatory gene in mammalian sex determination. The open reading frame of this gene contains an 80-amino-acid motif, the SRY-box, which shares a high degree of homology with a DNA-binding domain found in the high-mobility-group (HMG) proteins HMG1 and HMG2. The SRY box motif is highly conserved in several sequence-specific DNA-binding proteins that are known to act as transcription factors. Here we describe the use of degenerate PCR primers to identify SRY-related sequences containing the SRY-box motif from the genomic DNA of a variety of species. The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alligators and Crocodiles
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Evolution*
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Chickens
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DNA
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DNA-Binding Proteins / genetics*
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Drosophila
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Humans
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Lizards
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Molecular Sequence Data
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Nuclear Proteins*
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Polymerase Chain Reaction*
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Sequence Homology, Amino Acid
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Sex-Determining Region Y Protein
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Transcription Factors*
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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SRY protein, human
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Sex-Determining Region Y Protein
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Transcription Factors
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DNA
Associated data
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GENBANK/M86310
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GENBANK/M86311
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GENBANK/M86312
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GENBANK/M86313
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GENBANK/M86314
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GENBANK/M86315
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GENBANK/M86316
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GENBANK/M86317
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GENBANK/M86318
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GENBANK/M86319
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GENBANK/M86320
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GENBANK/M86321
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GENBANK/M86322
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GENBANK/M86323
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GENBANK/M86324
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GENBANK/M86325
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GENBANK/M86326
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GENBANK/M86327
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GENBANK/M86328
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GENBANK/M86329
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GENBANK/M86331
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GENBANK/M86332
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GENBANK/M86333
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GENBANK/M86334
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GENBANK/M86335
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GENBANK/M86336
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GENBANK/M86337
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GENBANK/M86338
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GENBANK/M86339