A DNA polymerase alpha-associated 56 kDa protein kinase

Biochem Biophys Res Commun. 1993 Jan 29;190(2):325-31. doi: 10.1006/bbrc.1993.1051.

Abstract

Initiation of chromosomal DNA replication must be carefully regulated during the cell cycle. We report that Drosophila embryo DNA polymerase alpha complex, isolated by immunological techniques, contains a protein kinase activity. The kinase will phosphorylate histone H1, but prefers peptides contained in the DNA polymerase alpha-kinase complex. Renaturation experiments determined that the kinase activity resides in a 56-kDa peptide.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Antibodies, Monoclonal
  • Autoradiography
  • Chromatography, Affinity
  • DNA Polymerase II / isolation & purification
  • DNA Polymerase II / metabolism*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology*
  • Electrophoresis, Polyacrylamide Gel
  • Histones / metabolism
  • Molecular Weight
  • Phosphorylation
  • Protein Denaturation
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Substrate Specificity

Substances

  • Antibodies, Monoclonal
  • Histones
  • Adenosine Triphosphate
  • Protein Kinases
  • DNA Polymerase II