Abstract
Initiation of chromosomal DNA replication must be carefully regulated during the cell cycle. We report that Drosophila embryo DNA polymerase alpha complex, isolated by immunological techniques, contains a protein kinase activity. The kinase will phosphorylate histone H1, but prefers peptides contained in the DNA polymerase alpha-kinase complex. Renaturation experiments determined that the kinase activity resides in a 56-kDa peptide.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Antibodies, Monoclonal
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Autoradiography
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Chromatography, Affinity
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DNA Polymerase II / isolation & purification
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DNA Polymerase II / metabolism*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / enzymology*
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Electrophoresis, Polyacrylamide Gel
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Histones / metabolism
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Molecular Weight
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Phosphorylation
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Protein Denaturation
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Protein Kinases / chemistry
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Protein Kinases / metabolism*
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Substrate Specificity
Substances
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Antibodies, Monoclonal
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Histones
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Adenosine Triphosphate
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Protein Kinases
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DNA Polymerase II