Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression

J Bacteriol. 1993 Jan;175(2):568-71. doi: 10.1128/jb.175.2.568-571.1993.

Abstract

DNA sequence and expressional analyses of the gcd gene of Escherichia coli K-12 W3110 revealed that two promoters that were detected were regulated negatively by cyclic AMP and positively by oxygen. Sequence conservation of the gcd gene between E. coli K-12 W3110 and PPA42 suggests that glucose dehydrogenase is required for the E. coli cells, even though it ordinarily exists as an apoprotein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Gene Expression Regulation, Bacterial*
  • Gene Expression Regulation, Enzymologic*
  • Genes, Bacterial*
  • Genotype
  • Glucose 1-Dehydrogenase
  • Glucose Dehydrogenases / genetics*
  • Glucose Dehydrogenases / metabolism
  • Kinetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides
  • Plasmids
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Transcription, Genetic

Substances

  • Oligodeoxyribonucleotides
  • Glucose Dehydrogenases
  • Glucose 1-Dehydrogenase

Associated data

  • GENBANK/D12651