Abstract
DNA sequence and expressional analyses of the gcd gene of Escherichia coli K-12 W3110 revealed that two promoters that were detected were regulated negatively by cyclic AMP and positively by oxygen. Sequence conservation of the gcd gene between E. coli K-12 W3110 and PPA42 suggests that glucose dehydrogenase is required for the E. coli cells, even though it ordinarily exists as an apoprotein.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Escherichia coli / enzymology*
-
Escherichia coli / genetics*
-
Gene Expression Regulation, Bacterial*
-
Gene Expression Regulation, Enzymologic*
-
Genes, Bacterial*
-
Genotype
-
Glucose 1-Dehydrogenase
-
Glucose Dehydrogenases / genetics*
-
Glucose Dehydrogenases / metabolism
-
Kinetics
-
Molecular Sequence Data
-
Mutagenesis, Site-Directed
-
Oligodeoxyribonucleotides
-
Plasmids
-
Polymerase Chain Reaction
-
Promoter Regions, Genetic
-
Transcription, Genetic
Substances
-
Oligodeoxyribonucleotides
-
Glucose Dehydrogenases
-
Glucose 1-Dehydrogenase