Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors

C Rojas; C Gutierrez; S Litvak; A Solari

doi:10.1016/0020-7519(93)90011-m

Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors

Int J Parasitol. 1993 May;23(3):361-4. doi: 10.1016/0020-7519(93)90011-m.

Authors

C Rojas¹, C Gutierrez, S Litvak, A Solari

Affiliation

¹ Departmento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.

PMID: 8395479
DOI: 10.1016/0020-7519(93)90011-m

Abstract

DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase inhibitors (aphidicolin, aracytidine, and dideoxythimidine) were chosen to study the nuclear and kinetoplast DNA synthesis in vivo. Inhibition was mainly observed with the nuclear DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aphidicolin / pharmacology
Autoradiography
Cytarabine / pharmacology
DNA, Circular / biosynthesis*
DNA, Kinetoplast
DNA, Protozoan / biosynthesis*
Dideoxynucleosides / pharmacology
Nucleic Acid Synthesis Inhibitors*
Trypanosoma cruzi / drug effects
Trypanosoma cruzi / genetics*

Substances

DNA, Circular
DNA, Kinetoplast
DNA, Protozoan
Dideoxynucleosides
Nucleic Acid Synthesis Inhibitors
Cytarabine
2',3'-dideoxythymidine
Aphidicolin