Activating transcription factor-1 can mediate Ca(2+)- and cAMP-inducible transcriptional activation

J Biol Chem. 1993 Mar 25;268(9):6714-20.

Abstract

Increased intracellular cAMP and Ca2+ levels can activate transcription of a number of eukaryotic genes through a common promoter element, the cAMP/Ca2+ response element. This element is the binding site for activating transcription factor (ATF)/CREB proteins, an extensive transcription factor family. Here we report that one member of this family, ATF-1, can mediate both Ca2+ and cAMP transcriptional responses, but that the responses to the two pathways differ in magnitude. In contrast, another family member, CREB, has been shown to mediate Ca2+ and cAMP responses to similar levels. Taken together, these results suggest a mechanism that allows cells to integrate and differentiate gene regulation by cAMP and Ca2+.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activating Transcription Factor 1
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium / metabolism*
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cyclic AMP / metabolism*
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • DNA-Binding Proteins*
  • Electrophoresis, Polyacrylamide Gel
  • Mice
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • PC12 Cells
  • Promoter Regions, Genetic
  • Protein Kinases / metabolism
  • Ribonucleases
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Tumor Cells, Cultured

Substances

  • Activating Transcription Factor 1
  • Atf1 protein, mouse
  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins
  • Oligodeoxyribonucleotides
  • Transcription Factors
  • Cyclic AMP
  • Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Ribonucleases
  • Calcium