Abstract
Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA, Mitochondrial / genetics*
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DNA, Mitochondrial / isolation & purification
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Electron Transport Complex IV / genetics*
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Restriction Mapping
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Sequence Homology, Nucleic Acid
Substances
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DNA, Mitochondrial
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Macromolecular Substances
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RNA, Messenger
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Electron Transport Complex IV
Associated data
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GENBANK/D13131
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GENBANK/D13132
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GENBANK/D13133
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GENBANK/D13134
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GENBANK/D13135
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GENBANK/D13136
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GENBANK/D13137
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GENBANK/U08372
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GENBANK/U09362
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GENBANK/Z14244