Abstract
The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Polymerase III / biosynthesis
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DNA Polymerase III / chemistry
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DNA Polymerase III / genetics*
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DNA Polymerase III / isolation & purification
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DNA, Bacterial
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Gene Expression Regulation, Bacterial
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Gene Expression Regulation, Enzymologic
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Genes, Bacterial*
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Molecular Sequence Data
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Open Reading Frames
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Plasmids
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Polymerase Chain Reaction
Substances
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DNA, Bacterial
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DNA Polymerase III