Abstract
We have previously used exon amplification to identify the ADD1 gene in cosmid Y24 from the Huntington's disease (HD) region of 4p16.3. The same technique has now yielded a second gene from this cosmid. This gene appears to encode a novel member of a superfamily of transporter proteins that includes active and passive transporters in a number of species. The predicted protein of 455 amino acids displays sequence similarity with the E. coli tetracycline resistance efflux protein encoded by cloning vector pBR322, and with a number of related transporters. This gene should open a route to isolating additional mammalian members of this growing superfamily.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calmodulin-Binding Proteins / genetics*
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Carrier Proteins / genetics*
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Chromosomes, Human, Pair 4*
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Escherichia coli / genetics*
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Exons
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Gene Library
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Genes*
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Genes, Bacterial
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Humans
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Membrane Transport Proteins / genetics*
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Molecular Sequence Data
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Multigene Family*
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Open Reading Frames
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Primates / genetics
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Repressor Proteins / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Tetracycline Resistance / genetics
Substances
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Calmodulin-Binding Proteins
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Carrier Proteins
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Membrane Transport Proteins
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Repressor Proteins
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TETRAN protein, human
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adducin
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tetracycline resistance-encoding transposon repressor protein