p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling

Bioconjug Chem. 1993 May-Jun;4(3):212-8. doi: 10.1021/bc00021a005.

Abstract

p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.

MeSH terms

  • Alkaline Phosphatase / chemistry
  • Amino Acid Sequence
  • Cross-Linking Reagents / chemical synthesis
  • Cross-Linking Reagents / chemistry*
  • Cyanates / chemical synthesis
  • Cyanates / chemistry*
  • Digoxigenin / chemistry
  • Drug Stability
  • Estradiol / chemistry
  • Haptens / chemistry
  • Maleimides / chemical synthesis
  • Maleimides / chemistry*
  • Molecular Sequence Data
  • Peptides / chemistry
  • Sulfhydryl Compounds / chemistry*
  • Vitamin B 12 / chemistry

Substances

  • Cross-Linking Reagents
  • Cyanates
  • Haptens
  • Maleimides
  • Peptides
  • Sulfhydryl Compounds
  • 4-maleimidophenyl isocyanate
  • Estradiol
  • Alkaline Phosphatase
  • Digoxigenin
  • Vitamin B 12