Abstract
p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.
MeSH terms
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Alkaline Phosphatase / chemistry
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Amino Acid Sequence
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Cross-Linking Reagents / chemical synthesis
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Cross-Linking Reagents / chemistry*
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Cyanates / chemical synthesis
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Cyanates / chemistry*
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Digoxigenin / chemistry
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Drug Stability
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Estradiol / chemistry
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Haptens / chemistry
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Maleimides / chemical synthesis
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Maleimides / chemistry*
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Molecular Sequence Data
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Peptides / chemistry
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Sulfhydryl Compounds / chemistry*
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Vitamin B 12 / chemistry
Substances
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Cross-Linking Reagents
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Cyanates
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Haptens
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Maleimides
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Peptides
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Sulfhydryl Compounds
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4-maleimidophenyl isocyanate
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Estradiol
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Alkaline Phosphatase
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Digoxigenin
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Vitamin B 12