Misincorporation of ribonucleotides by DNA polymerase during in vitro DNA replication

Nucleic Acids Symp Ser. 1993:(29):133-4.

Abstract

The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.

MeSH terms

  • Bacteriophage M13 / genetics
  • Base Composition
  • Base Sequence
  • DNA Polymerase I / metabolism*
  • DNA Replication*
  • DNA, Viral / biosynthesis*
  • Escherichia coli / enzymology
  • Molecular Sequence Data
  • Ribonucleotides / metabolism*
  • Templates, Genetic

Substances

  • DNA, Viral
  • Ribonucleotides
  • DNA Polymerase I