Abstract
POU-domain transcription factors are known as developmental regulators which control organ development and cell phenotypes. In order to clarify the roles of POU-domain transcription factors in cell differentiation, we cloned a novel POU family gene, Epoc-1, from a murine thymus cDNA library. The amino acid (aa) sequence of the POU-specific domain of Epoc-1 is almost identical to those of Oct-1 and Oct-2. However, within the POU-homeodomain, 13 out of 60 aa differ between Epoc-1 and Oct-2. Recombinant Epoc-1 products were found to bind specifically to the octamer sequence. Epoc-1 was found to be expressed in skin, thymus, stomach and testis. In situ hybridization experiments and RNase protection assays indicated that Epoc-1 is expressed in the epidermal basal cells of the skin, which contain stem cells unipotent for keratinocyte differentiation and in thymic stromal elements. These results suggest that Epoc-1 might be one of the developmental regulators which controls epidermal development and thymic organogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Differentiation / genetics
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Cloning, Molecular
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DNA
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Epidermal Cells
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Epidermis / metabolism
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Gene Expression
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Host Cell Factor C1
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Mice
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Molecular Sequence Data
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Multigene Family
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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Octamer Transcription Factors
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Organ Specificity
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POU Domain Factors
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Sequence Homology, Amino Acid
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Stromal Cells / metabolism
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Thymus Gland / cytology
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Thymus Gland / metabolism*
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Transcription Factors / biosynthesis
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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DNA-Binding Proteins
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Hcfc1 protein, mouse
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Host Cell Factor C1
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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Octamer Transcription Factors
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POU Domain Factors
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POU2F3 protein, human
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Pou2f1 protein, mouse
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Pou2f2 protein, mouse
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Pou2f3 protein, mouse
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Transcription Factors
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DNA
Associated data
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GENBANK/D38370
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GENBANK/D38371
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GENBANK/L08645
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GENBANK/L14677
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GENBANK/L24524
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GENBANK/L24525
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GENBANK/L24526
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GENBANK/L24527
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GENBANK/L24528
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GENBANK/L24529