Localization of a human receptor tyrosine kinase (ETK1) to chromosome region 3p11.2

I P Wicks; N M Lapsys; E Baker; L J Campbell; A W Boyd; G R Sutherland

doi:10.1006/geno.1994.1009

Localization of a human receptor tyrosine kinase (ETK1) to chromosome region 3p11.2

Genomics. 1994 Jan 1;19(1):38-41. doi: 10.1006/geno.1994.1009.

Authors

I P Wicks¹, N M Lapsys, E Baker, L J Campbell, A W Boyd, G R Sutherland

Affiliation

¹ Lions Laboratory, Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.

PMID: 8188238
DOI: 10.1006/geno.1994.1009

Abstract

We have recently described a human receptor tyrosine kinase (hek) that is expressed by some pre-B and thymic T cell lines, but is not detectable on normal adult human tissues. Gene cloning studies established that hek is a new member of the EPH family of receptor tyrosine kinases. The expression of hek may normally be developmentally regulated and inappropriate expression may contribute to oncogenesis. In the present study, we have used Southern blot analysis of somatic cell hybrids and fluorescence in situ hybridization to localize the hek gene to human chromosome region 3p11.2. Karyotype analysis of the cell lines that over-express hek showed no cytogenetically visible abnormality involving the hek locus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 3*
Cricetinae
DNA, Complementary / genetics
Enzyme Induction
Gene Expression Regulation, Neoplastic
Genes*
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Mice
Multigene Family
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics
Receptor Protein-Tyrosine Kinases / biosynthesis
Receptor Protein-Tyrosine Kinases / genetics*
Receptor, EphA3
Receptors, Cell Surface / biosynthesis
Receptors, Cell Surface / genetics*
Tumor Cells, Cultured

Substances

DNA, Complementary
Neoplasm Proteins
Receptors, Cell Surface
Receptor Protein-Tyrosine Kinases
Receptor, EphA3