Crystallization of DNA polymerase II from Escherichia coli

J Mol Biol. 1994 Apr 22;238(1):120-2. doi: 10.1006/jmbi.1994.1765.

Abstract

DNA polymerase II of Escherichia coli, an alpha-like or group B polymerase, has been crystallized. The crystals are orthorhombic, space group P2(1)2(1)2, with cell dimensions a = 94.4 A, b = 118.2 A, c = 84.2 A and diffract to at least 3.0 A resolution. This is the first example of a group B polymerase to be crystallized.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • DNA Polymerase II / chemistry
  • DNA Polymerase II / isolation & purification*
  • Escherichia coli / enzymology*

Substances

  • DNA Polymerase II