Genomic structure of the POU-related hepatic transcription factor HNF-1 alpha

Biol Chem Hoppe Seyler. 1993 Jul;374(7):419-25. doi: 10.1515/bchm3.1993.374.7-12.419.

Abstract

Hepatocyte Nuclear Factor-1 alpha (HNF-1 alpha) is a vertebrate transcriptional regulatory protein which plays a prominent role in the activation of a large family of hepatocyte-specific genes. It is the prototype of a new subfamily of POU-related proteins important in embryogenesis and cell-type specification. Here we report on the genomic structure and DNA sequence of HNF-1 alpha of the hen. Functional domains are non-uniformly conserved between hen and mammals consistent with the notion of functions in the hen oviduct that were lost in mammals after avian mammalian separation. The unusual homeo-related DNA-binding domain is well preserved between birds and mammals. The exon/intron pattern of the gene changed during evolution. The rat gene consists of 9 exons, the chicken gene of 10. In mammals one intron was eliminated that in hens subdivides the serine-rich transactivation domain. Comparison of the sequences at this junction indicates the involvement of elements of the V(D)J recombination system in the process of intron elimination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens
  • DNA-Binding Proteins / chemistry*
  • Exons
  • Genes
  • Genes, Homeobox
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-alpha
  • Hepatocyte Nuclear Factor 1-beta
  • Introns
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Rats
  • Transcription Factors / chemistry*

Substances

  • DNA-Binding Proteins
  • Hepatocyte Nuclear Factor 1-alpha
  • Hnf1a protein, rat
  • Nuclear Proteins
  • Transcription Factors
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-beta

Associated data

  • GENBANK/L08443
  • GENBANK/S67527
  • GENBANK/X67689
  • GENBANK/X67690
  • GENBANK/X72737
  • GENBANK/X72738
  • GENBANK/X72739
  • GENBANK/X72740
  • GENBANK/X72741
  • GENBANK/X72742