Alternative splicing of human T-cell-specific MAL mRNA and its correlation with the exon/intron organization of the gene

Genomics. 1994 May 15;21(2):447-50. doi: 10.1006/geno.1994.1294.

Abstract

Sequence analysis of the T-cell-specific MAL gene revealed four exons, each encoding a hydrophobic, presumably membrane-associated, segment and its adjacent hydrophilic sequence. Amplification by the polymerase chain reaction of cDNA from different T-cell samples indicated the existence of four different forms of MAL mRNA, termed MAL-a, -b, -c, and -d, that arise from differential usage of exons II and/or III. As the three introns were located between complete codons, the reading frame was maintained in all the transcripts. A model resembling the structures postulated for different proteolipid proteins is proposed for the protein encoded by each alternative mRNA species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Base Sequence
  • Cell Line
  • DNA Primers
  • Exons
  • Humans
  • Introns
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Myelin Proteins*
  • Myelin and Lymphocyte-Associated Proteolipid Proteins
  • Polymerase Chain Reaction
  • Protein Biosynthesis*
  • Proteins / genetics
  • Proteolipids*
  • RNA, Messenger / metabolism*
  • Restriction Mapping
  • T-Lymphocytes / metabolism*
  • Transcription, Genetic

Substances

  • DNA Primers
  • MAL protein, human
  • Membrane Transport Proteins
  • Myelin Proteins
  • Myelin and Lymphocyte-Associated Proteolipid Proteins
  • Proteins
  • Proteolipids
  • RNA, Messenger

Associated data

  • GENBANK/X76220
  • GENBANK/X76221
  • GENBANK/X76222
  • GENBANK/X76223
  • GENBANK/X76678
  • GENBANK/X76679
  • GENBANK/X76680
  • GENBANK/X76681