Abstract
Sequence analysis of the T-cell-specific MAL gene revealed four exons, each encoding a hydrophobic, presumably membrane-associated, segment and its adjacent hydrophilic sequence. Amplification by the polymerase chain reaction of cDNA from different T-cell samples indicated the existence of four different forms of MAL mRNA, termed MAL-a, -b, -c, and -d, that arise from differential usage of exons II and/or III. As the three introns were located between complete codons, the reading frame was maintained in all the transcripts. A model resembling the structures postulated for different proteolipid proteins is proposed for the protein encoded by each alternative mRNA species.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alternative Splicing*
-
Base Sequence
-
Cell Line
-
DNA Primers
-
Exons
-
Humans
-
Introns
-
Membrane Transport Proteins*
-
Molecular Sequence Data
-
Myelin Proteins*
-
Myelin and Lymphocyte-Associated Proteolipid Proteins
-
Polymerase Chain Reaction
-
Protein Biosynthesis*
-
Proteins / genetics
-
Proteolipids*
-
RNA, Messenger / metabolism*
-
Restriction Mapping
-
T-Lymphocytes / metabolism*
-
Transcription, Genetic
Substances
-
DNA Primers
-
MAL protein, human
-
Membrane Transport Proteins
-
Myelin Proteins
-
Myelin and Lymphocyte-Associated Proteolipid Proteins
-
Proteins
-
Proteolipids
-
RNA, Messenger
Associated data
-
GENBANK/X76220
-
GENBANK/X76221
-
GENBANK/X76222
-
GENBANK/X76223
-
GENBANK/X76678
-
GENBANK/X76679
-
GENBANK/X76680
-
GENBANK/X76681