Cloning and analysis of MAGE-1-related genes

Biochem Biophys Res Commun. 1994 Jul 15;202(1):549-55. doi: 10.1006/bbrc.1994.1963.

Abstract

The spectrum of MAGE gene expression in the human melanoma cell line DM150 was examined using reverse transcription polymerase chain reaction and cDNA cloning. We have isolated five full-length cDNAs from DM150 which were identified as MAGE-1, MAGE-3, MAGE-12 and two previously undescribed MAGE genes, MAGE-3b and MAGE-X2. DNA sequence analysis of the coding regions of the MAGE-3b and MAGE-X2 genes revealed 83% and 88% identity with MAGE-1, while MAGE-3b was 98% homologous with the full length MAGE-3 clone. The predicted amino acid sequences of MAGE-X2 and MAGE-3b contain consensus HLA-A1 peptide binding motifs, suggesting that, like MAGE-1, they may code for tumor-associated antigens. In addition, a nonamer peptide encoded by both the MAGE-3 and MAGE-12 genes was shown by direct binding studies to contain an aggretope for HLA-A2.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • CHO Cells
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • DNA Primers
  • DNA, Complementary / biosynthesis
  • Humans
  • Melanoma
  • Melanoma-Specific Antigens
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Polymerase Chain Reaction / methods
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • DNA Primers
  • DNA, Complementary
  • MAGEA1 protein, human
  • Melanoma-Specific Antigens
  • Neoplasm Proteins