Identification and elimination of DNA sequences in Taq DNA polymerase

M S Hughes; L A Beck; R A Skuce

doi:10.1128/jcm.32.8.2007-2008.1994

Identification and elimination of DNA sequences in Taq DNA polymerase

J Clin Microbiol. 1994 Aug;32(8):2007-8. doi: 10.1128/jcm.32.8.2007-2008.1994.

Authors

M S Hughes¹, L A Beck, R A Skuce

Affiliation

¹ Department of Agriculture for Northern Ireland, Stromont, Belfast.

Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

Publication types

Comparative Study

MeSH terms

Base Sequence
DNA, Bacterial / analysis*
DNA, Bacterial / drug effects
DNA, Bacterial / radiation effects
DNA-Directed DNA Polymerase / isolation & purification*
Drug Contamination*
Methoxsalen / pharmacology
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Taq Polymerase
Ultraviolet Rays

Substances

DNA, Bacterial
Taq Polymerase
DNA-Directed DNA Polymerase
Methoxsalen