Abstract
Recent studies with 8-oxodeoxyguanosine triphosphate (8-oxodGTP) have suggested that incorporation of oxidized nucleotides from the precursor pool into DNA may have deleterious effects. Here we show that 5-hydroxydeoxycytosine triphosphate (5-OHdCTP) and 5-hydroxydeoxyuridine triphosphate (5-OHdUTP) are more efficient substrates than 8-oxodGTP for Escherichia coli DNA polymerase I Klenow fragment lacking proofreading activity, while 8-oxodeoxyadenosine triphosphate (8-oxodGTP, 5-OHdCTP can mispair with dA in DNA but with lower efficiency. Since the 5-hydroxypyrimidines are present in normal and oxidized cellular DNA in amounts similar to the 8-oxopurines, these data suggest that enzymatic mechanisms might exist for removing them from the DNA precursor pools.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA / biosynthesis*
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DNA Polymerase I / metabolism*
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Deoxyadenine Nucleotides / metabolism
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Deoxycytosine Nucleotides / chemical synthesis
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Deoxycytosine Nucleotides / metabolism*
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Deoxyguanine Nucleotides / chemical synthesis
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Deoxyguanine Nucleotides / metabolism*
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Deoxyuracil Nucleotides / chemical synthesis
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Deoxyuracil Nucleotides / metabolism*
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Kinetics
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Molecular Sequence Data
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Templates, Genetic
Substances
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2'-deoxy-5-hydroxycytosine triphosphate
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8-oxodeoxyadenosine triphosphate
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Deoxyadenine Nucleotides
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Deoxycytosine Nucleotides
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Deoxyguanine Nucleotides
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Deoxyuracil Nucleotides
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8-oxodeoxyguanosine triphosphate
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2'-deoxy-5-hydroxyuridine triphosphate
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DNA
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DNA Polymerase I