Your browser version may not work well with NCBI's Web applications. More information here...
1: DNA Seq. 1994;5(1):57-61.Links

Nucleotide sequence of a gene for nitrite reductase from Arabidopsis thaliana.

Tanaka T, Ida S, Irifune K, Oeda K, Morikawa H.

Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.

A nitrite reductase (NiR) gene was recovered from Arabidopsis thaliana genomic library by the homology with a cDNA of spinach NiR and sequenced. Based on the comparison with the spinach cDNA, the Arabidopsis NiR gene was concluded to contain 4 exons [exon 1 of 376 bp (beginning with ATG start codon), exon 2 of 355 bp, exon 3 of 289 bp and exon 4 of 741 bp (ending at TGA stop codon)] and 3 introns (intron 1 of 196 bp, intron 2 of 81 bp and intron 3 of 77 bp). This conclusion was confirmed by the analysis using the RT-PCR method. The deduced amino acid sequence of the coding region of the Arabidopsis NiR gene had high similarities with those of NiR genes of other plants including spinach.

PMID: 7894060 [PubMed - indexed for MEDLINE]

Related Articles