Head-on collision between a DNA replication apparatus and RNA polymerase transcription complex

Science. 1995 Feb 24;267(5201):1131-7. doi: 10.1126/science.7855590.

Abstract

An in vitro system reconstituted from purified proteins has been used to examine what happens when the DNA replication apparatus of bacteriophage T4 collides with an Escherichia coli RNA polymerase ternary transcription complex that is poised to move in the direction opposite to that of the moving replication fork. In the absence of a DNA helicase, the replication fork stalls for many minutes after its encounter with the RNA polymerase. However, when the T4 gene 41 DNA helicase is present, the replication fork passes the RNA polymerase after a pause of a few seconds. This brief pause is longer than the pause observed for a codirectional collision between the same two polymerases, suggesting that there is an inherent disadvantage to having replication and transcription directions oriented head to head. As for a codirectional collision, the RNA polymerase remains competent to resume faithful RNA chain elongation after the DNA replication fork passes; most strikingly, the RNA polymerase has switched from its original template strand to use the newly synthesized daughter DNA strand as the template.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophage T4
  • Base Sequence
  • DNA Helicases / metabolism
  • DNA Replication*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • DNA, Circular / metabolism
  • DNA, Viral / biosynthesis
  • DNA, Viral / genetics
  • DNA-Directed DNA Polymerase / metabolism
  • DNA-Directed RNA Polymerases / metabolism*
  • Escherichia coli
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis*
  • Replicon / physiology*
  • Templates, Genetic
  • Transcription, Genetic*

Substances

  • DNA, Bacterial
  • DNA, Circular
  • DNA, Viral
  • RNA, Messenger
  • DNA-Directed RNA Polymerases
  • DNA-Directed DNA Polymerase
  • DNA Helicases