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1: Biochem J. 1995 Jan 15;305 ( Pt 2):363-6.Click here to read Links

Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B.

Emiliani C, Martino S, Stirling JL, Maras B, Orlacchio A.

Dipartimento di Biologia Cellulare e Molecolare, Università degli Studi di Perugia, Italy.

Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.

PMID: 7832746 [PubMed - indexed for MEDLINE]

PMCID: PMC1136369

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