Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene

J Biol Chem. 1995 Jan 13;270(2):511-4. doi: 10.1074/jbc.270.2.511.

Abstract

The megakaryocyte growth and development factor (MGDF) is a cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. In this study, we describe the genomic structure of the human MGDF gene. The MGDF gene was found to consist of seven exons and six introns spanning 8 kilobases. The protein is encoded by exons 3 through 7. The human MGDF gene has been mapped to chromosome 3q26.3. In addition to the previously described full-length cDNA, two cDNA variants were isolated from human fetal liver. Comparison of these two cDNA sequences with the genomic sequence indicates that they arise by differential splicing.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Megakaryocytes / metabolism*
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Thrombopoietin / genetics*
  • Thrombopoietin / metabolism

Substances

  • DNA, Complementary
  • Thrombopoietin

Associated data

  • GENBANK/U17071