Abstract
We have previously reported the cloning of stem cell tyrosine kinase-1 (STK-1, gene symbol FLT3), the human homolog of the mouse Flk2/Flt3 gene (Small et al., 1994). This growth factor receptor is expressed in CD34+ human bone marrow (stem/progenitor) cells. We have mapped the gene for STK-1 using PCR screening of a human-rodent somatic cell hybrid panel, and fluorescence in situ molecular hybridization (FISH) of STK-1 genomic probes to mitotic chromosomes of normal human lymphocytes. The results of both analyses show that the gene for STK-1 (FLT3) localizes to chromosome 13q12-->q13.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Base Sequence
-
Bone Marrow
-
Chromosome Mapping
-
Chromosomes, Human, Pair 13*
-
DNA Primers
-
Hematopoietic Stem Cells / metabolism
-
Humans
-
Hybrid Cells
-
In Situ Hybridization, Fluorescence
-
Mice
-
Molecular Sequence Data
-
Polymerase Chain Reaction
-
Proto-Oncogene Proteins / genetics*
-
Receptor Protein-Tyrosine Kinases / genetics*
-
Receptors, Cell Surface / genetics
-
fms-Like Tyrosine Kinase 3
Substances
-
DNA Primers
-
Proto-Oncogene Proteins
-
Receptors, Cell Surface
-
FLT3 protein, human
-
Flt3 protein, mouse
-
Receptor Protein-Tyrosine Kinases
-
fms-Like Tyrosine Kinase 3