Localization of the human stem cell tyrosine kinase-1 gene (FLT3) to 13q12-->q13

Cytogenet Cell Genet. 1995;70(3-4):255-7. doi: 10.1159/000134046.

Abstract

We have previously reported the cloning of stem cell tyrosine kinase-1 (STK-1, gene symbol FLT3), the human homolog of the mouse Flk2/Flt3 gene (Small et al., 1994). This growth factor receptor is expressed in CD34+ human bone marrow (stem/progenitor) cells. We have mapped the gene for STK-1 using PCR screening of a human-rodent somatic cell hybrid panel, and fluorescence in situ molecular hybridization (FISH) of STK-1 genomic probes to mitotic chromosomes of normal human lymphocytes. The results of both analyses show that the gene for STK-1 (FLT3) localizes to chromosome 13q12-->q13.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13*
  • DNA Primers
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / genetics*
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptors, Cell Surface / genetics
  • fms-Like Tyrosine Kinase 3

Substances

  • DNA Primers
  • Proto-Oncogene Proteins
  • Receptors, Cell Surface
  • FLT3 protein, human
  • Flt3 protein, mouse
  • Receptor Protein-Tyrosine Kinases
  • fms-Like Tyrosine Kinase 3