Isolation, sequence and expression in Escherichia ...[Plant Mol Biol. 1995]

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1: Plant Mol Biol. 1995 Mar;27(5):1037-42.Links

Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum.

Merchán F, Prieto R, Kindle KL, Llama MJ, Serra JL, Fernández E.

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Spain.

The nitrite reductase (NiR) gene (nirA) has been isolated and sequenced from the filamentous, thermophilic non-N2-fixing cyanobacterium Phormidium laminosum. Putative promoter-like and Shine-Dalgarno sequences appear at the 5' end of the 1533 bp long nir-coding region. The deduced amino acid sequence of NiR from P. laminosum corresponds to a 56 kDa polypeptide, a size identical to the molecular mass previously determined for the pure enzyme, and shows a high identity with amino acid sequences from ferredoxin-dependent NiR. This cyanobacterial NiR gene has been efficiently expressed in Escherichia coli DH5 alpha from the E. coli lac promoter and probably from the P. laminosum NiR promoter.

PMID: 7766873 [PubMed - indexed for MEDLINE]

Cloning and sequencing of the nitrate transport system from the thermophilic, filamentous cyanobacterium Phormidium laminosum: comparative analysis with the homologous system from Synechococcus sp. PCC 7942. [Plant Mol Biol. 1995]
A novel nitrite reductase gene from the cyanobacterium Plectonema boryanum. [J Bacteriol. 1995]
Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum. [Plant Mol Biol. 1995]
Expression of spinach nitrite reductase in Escherichia coli: site-directed mutagenesis of predicted active site amino acids. [Arch Biochem Biophys. 1995]
Cloning and characterization of a nitrite reductase gene from Alcaligenes faecalis and its expression in Escherichia coli. [J Gen Microbiol. 1993]

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Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum.

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