Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB)

Genomics. 1994 Dec;24(3):440-4. doi: 10.1006/geno.1994.1650.

Abstract

Two human clathrin light-chain genes have been defined. The gene (CLTA) encoding the LCa light chain maps to the long arm of chromosome 12 at 12q23-q24 and that encoding the LCb light chain (CLTB) maps to the long arm of chromosome 4 at 4q2-q3. Isolation and characterization of partial genomic clones encoding human LCa and LCb reveal the neuron-specific insertions of the LCa and LCb proteins to be encoded by discrete exons, thus proving that clathrin light chains undergo alternate mRNA splicing to generate tissue-specific protein isoforms. The insertion sequence of LCb is encoded by a single exon and that of LCa by two exons. The first of the two neuron-specific LCa exons is homologous to the corresponding LCb exon. An intronic sequence of the LCb gene with similarity to the second neuron-specific exon of the LCa gene has been identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12
  • Chromosomes, Human, Pair 4
  • Clathrin / chemistry
  • Clathrin / genetics*
  • Cloning, Molecular
  • Consensus Sequence
  • DNA / genetics
  • DNA, Complementary / genetics
  • Exons
  • Humans
  • Hybrid Cells
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Neurons / metabolism
  • RNA Splicing / genetics
  • Repetitive Sequences, Nucleic Acid

Substances

  • Clathrin
  • DNA, Complementary
  • DNA

Associated data

  • GENBANK/X81636
  • GENBANK/X81637