Continued expression of the chicken caudal homologue in endodermally derived organs

Dev Biol. 1993 Mar;156(1):155-63. doi: 10.1006/dbio.1993.1066.

Abstract

We show that the CHox-cad gene is continuously expressed throughout embryogenesis from Days 1 to 14 of development. In the adult chicken, CHox-cad appears to be expressed exclusively in the gut. Genomic Southern blot hybridization experiments suggest that there may be only a single CHox-cad gene per haploid genome from which RNAs of 2.9 and 1.6 kb are transcribed. In situ hybridization to sections of 80-hr to 12-day chick embryos with a CHox-cad probe reveals that this gene is expressed at high levels in organs of endodermal origin: the developing liver, pancreas, lung, and in the epithelial lining of the entire intestine. By Day 16 of development, CHox-cad signals are observed in the epithelial lining of the gut but no longer in the other endodermally derived organs. The coincidence of CHox-cad expression with the onset of development of these endoderm-derived organs may indicate an important role for that gene during the early steps of organogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Southern
  • Chick Embryo / physiology*
  • Chickens / genetics*
  • DNA / genetics
  • DNA / isolation & purification
  • Endoderm / physiology*
  • Genes, Homeobox*
  • Genome
  • Genomic Library
  • Haploidy
  • In Situ Hybridization
  • Introns
  • Molecular Sequence Data
  • Open Reading Frames
  • Organ Specificity
  • Poly A / genetics
  • Poly A / isolation & purification
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Poly A
  • RNA
  • DNA

Associated data

  • GENBANK/S54400
  • GENBANK/S54402
  • GENBANK/S54403
  • GENBANK/S54406
  • GENBANK/S54407
  • GENBANK/S54408
  • GENBANK/S54409
  • GENBANK/S54411
  • GENBANK/S54556
  • GENBANK/X69129