Abstract
We have identified an antigen recognized on a human melanoma by autologous cytolytic T lymphocytes. It is encoded by a gene that is expressed in many normal tissues. Remarkably, the sequence coding for the antigenic peptide is located across an exon-intron junction. A point mutation is present in the intron that generates an amino acid change that is essential for the recognition of the peptide by the anti-tumor cytotoxic T lymphocytes. This observation suggests that the T-cell-mediated surveillance of the integrity of the genome may extend to some intronic regions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Antigens, Neoplasm / genetics
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Antigens, Neoplasm / immunology*
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Base Sequence
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Binding, Competitive
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Cell Line
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Cloning, Molecular
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Cytotoxicity, Immunologic
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Exons
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Humans
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Introns*
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Kinetics
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Melanoma / immunology*
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Molecular Sequence Data
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Mutagenesis
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Neoplasm Proteins / immunology*
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Point Mutation*
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Recombinant Proteins / immunology
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T-Lymphocytes, Cytotoxic / immunology*
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Transfection
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Tumor Cells, Cultured
Substances
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Antigens, Neoplasm
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Neoplasm Proteins
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Recombinant Proteins
Associated data
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GENBANK/U20896
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GENBANK/U20897
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GENBANK/U20908