A mutated intron sequence codes for an antigenic peptide recognized by cytolytic T lymphocytes on a human melanoma

Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7976-80. doi: 10.1073/pnas.92.17.7976.

Abstract

We have identified an antigen recognized on a human melanoma by autologous cytolytic T lymphocytes. It is encoded by a gene that is expressed in many normal tissues. Remarkably, the sequence coding for the antigenic peptide is located across an exon-intron junction. A point mutation is present in the intron that generates an amino acid change that is essential for the recognition of the peptide by the anti-tumor cytotoxic T lymphocytes. This observation suggests that the T-cell-mediated surveillance of the integrity of the genome may extend to some intronic regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / immunology*
  • Base Sequence
  • Binding, Competitive
  • Cell Line
  • Cloning, Molecular
  • Cytotoxicity, Immunologic
  • Exons
  • Humans
  • Introns*
  • Kinetics
  • Melanoma / immunology*
  • Molecular Sequence Data
  • Mutagenesis
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology*
  • Point Mutation*
  • Recombinant Proteins / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • Neoplasm Proteins
  • Recombinant Proteins

Associated data

  • GENBANK/U20896
  • GENBANK/U20897
  • GENBANK/U20908