Abstract
We have isolated cDNA clone encoding a protein that can associate with Id, a helix-loop-helix (HLH) protein. This protein is named MIDA1 (Mouse Id Associate 1), and its predicted amino acid sequence consists of Zuotin (a putative Z-DNA binding protein in yeast) homology region and tryptophan-mediated repeats similar to c-Myb oncoprotein. MIDA1 associates with the HLH region of Id with the conserved region adjacent to eukaryotic DnaJ conserved motif within the Zuotin homology region, although it does not have any canonical HLH motif. The addition of antisense oligonucleotide of MIDA1 inhibited growth of murine erythroleukemia cells without interfering with erythroid differentiation, indicating that it regulates cell growth.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Cell Division
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
DNA-Binding Proteins / physiology*
-
Fungal Proteins / chemistry
-
Helix-Loop-Helix Motifs*
-
Humans
-
Inhibitor of Differentiation Protein 1
-
Mice
-
Molecular Chaperones
-
Molecular Sequence Data
-
Oligonucleotides, Antisense / pharmacology
-
Repressor Proteins*
-
Saccharomyces cerevisiae Proteins*
-
Sequence Homology, Amino Acid
-
Transcription Factors*
-
Transfection
-
Tumor Cells, Cultured
Substances
-
DNA-Binding Proteins
-
Fungal Proteins
-
ID1 protein, human
-
Idb1 protein, mouse
-
Inhibitor of Differentiation Protein 1
-
Molecular Chaperones
-
Oligonucleotides, Antisense
-
Repressor Proteins
-
Saccharomyces cerevisiae Proteins
-
Transcription Factors
-
ZUO1 protein, S cerevisiae
-
Zrf2 protein, mouse