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1: Biochim Biophys Acta. 1994 Nov 16;1209(1):107-10.Click here to read Links

Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA.

McCann KP, Akbari MT, Williams AC, Ramsden DB.

Department of Clinical Neurology, University of Birmingham, Queen Elizabeth Hospital, UK.

The base sequence of cDNA encoding the complete human liver cysteine dioxygenase type I (CDO-I; EC 1.13.11.20) message, and the derived amino-acid sequence are reported. CDO-I is encoded on a single mRNA species (approx. 1.5 kb). Human CDO-I clones were identified by screening a liver cDNA library, and inserts were isolated and sequenced. In addition, human liver total RNA was reverse-transcribed and CDO-I cDNA amplified by PCR using a modified poly-T primer and specific CDO-I primers to give a second source of sequencing template. The CDO-I message encodes a 200 amino-acid residue protein, the sequence of which has greater than 90% homology with the equivalent rat enzyme. The message was not expressed in a human hepatoma cell line (Hep G2).

PMID: 7524679 [PubMed - indexed for MEDLINE]

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