Abstract
We have analyzed recombinant human immunodeficiency virus type 1 reverse transcriptases that contain mutations at position 184. These variants retain high levels of RNA-dependent DNA polymerase activity and show resistance to ddITP. However, the mutants varied in their ability to polymerize processively. The variants Met184Ile and Met184Val showed slight reductions in processivity relative to that of the wild-type enzyme; the variants Met184Ala and Met184Leu showed considerable reductions in their processivity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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DNA Mutational Analysis
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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HIV Reverse Transcriptase
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Molecular Sequence Data
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Mutagenesis, Insertional
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Mutation*
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RNA-Directed DNA Polymerase / genetics*
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RNA-Directed DNA Polymerase / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Recombinant Proteins
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase
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DNA-Directed DNA Polymerase