Abstract
Using a mouse cDNA probe we have identified a human uridine phosphorylase cDNA clone from the cDNA library of a human colorectal tumor cell line, HCT116. The recombinant human uridine phosphorylase expressed in COS-7 cells demonstrated specific enzyme activity with uridine as the substrate; this activity was inhibited by the competitive inhibitor 2,2'-anhydro-5-ethyluridine. Northern blot analysis with the cDNA as a probe demonstrated high levels of mRNA expression in several tumor cell lines but very low level in normal cell, WI-38. The expression of uridine phosphorylase mRNA in HCT-116 cells was further enhanced by treating the cells with vitamin D3 and the inflammatory cytokines: tumor necrosis factor alpha, interleukin 1 alpha and interferon gamma.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcitriol / pharmacology
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Cell Line
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Chlorocebus aethiops
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Cloning, Molecular
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Colorectal Neoplasms
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Cytokines / pharmacology
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DNA Probes
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DNA, Complementary
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Gene Expression* / drug effects
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Humans
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Interferon-gamma / pharmacology
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Interleukin-1 / pharmacology
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Kinetics
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Mice
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Molecular Sequence Data
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RNA, Messenger / biosynthesis
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Reference Values
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / pharmacology
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Uridine Phosphorylase / biosynthesis*
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Uridine Phosphorylase / chemistry
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Uridine Phosphorylase / metabolism
Substances
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Cytokines
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DNA Probes
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DNA, Complementary
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Interleukin-1
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RNA, Messenger
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Interferon-gamma
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Uridine Phosphorylase
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Calcitriol