Cloning, nucleotide sequencing, and expression of ...[Appl Environ Microbiol. 1995]

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1: Appl Environ Microbiol. 1995 Aug;61(8):3169-71. Links

Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.

Dairi T, Asano Y.

Biotechnology Research Center, Toyama Prefectural University, Japan.

The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).

PMID: 7487048 [PubMed - indexed for MEDLINE]

PMCID: PMC167592

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Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.

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