Abstract
We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ampholyte Mixtures / pharmacology
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Animals
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Cattle
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DNA Polymerase II / antagonists & inhibitors
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DNA Polymerase II / isolation & purification*
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DNA Polymerase II / metabolism
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DNA-Directed DNA Polymerase / isolation & purification*
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Ethylmaleimide / pharmacology
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Molecular Weight
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Poly dA-dT
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Protein Conformation
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Substrate Specificity
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Thymus Gland / enzymology*
Substances
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Ampholyte Mixtures
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Poly dA-dT
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DNA Polymerase II
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DNA-Directed DNA Polymerase
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Ethylmaleimide