[Affinity modification of Escherichia coli Dna-polymerase I by 4-(N-2-chloroethyl-N-methylamino)-benzyl-gamma-amides of dTTP and dATP]

V N Buneva; T V Demidova; D G Knorre; N V Kudriashova; A G Romashchenko

[Affinity modification of Escherichia coli Dna-polymerase I by 4-(N-2-chloroethyl-N-methylamino)-benzyl-gamma-amides of dTTP and dATP]

Mol Biol (Mosk). 1980 Sep-Oct;14(5):1080-7.

[Article in Russian]

Authors

V N Buneva, T V Demidova, D G Knorre, N V Kudriashova, A G Romashchenko

PMID: 6999329

Abstract

The interaction of dTTP and dATP gamma-4-(N-2-chloroethyl-N-methylamino) benzylamidates with E. coli DNA-polymerase I were studied. dTTP and dATP gamma-4-(N-2-hydroxyethyl-N-methylamino) benzylamidates act as competitive inhibitors of DNA-polymerase I. Ki values for gamma-analogues of dTTP and dATP have been determined. Reactive dTTP and dATP derivatives are shown to be affinity reagent for this enzyme.

Publication types

Comparative Study

MeSH terms

Adenosine Triphosphate / analogs & derivatives*
Chemical Phenomena
Chemistry
DNA Polymerase I* / antagonists & inhibitors
DNA-Directed DNA Polymerase*
Escherichia coli / enzymology*
Thymine Nucleotides*

Substances

Thymine Nucleotides
4-(N-(2-chloroethyl)-N-methylamino)benzyl-gamma-amide dTTP
4-(N-2-chloroethyl-N-methylamino)benzyl-gamma-amide dATP
Adenosine Triphosphate
DNA Polymerase I
DNA-Directed DNA Polymerase