Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA

J Biol Chem. 1977 Jul 25;252(14):5040-53.

Abstract

Sequences of human beta-globin mRNA were determined by analysis of complementary DNA. beta-mRNA was transcribed into double-stranded cDNA by RNA-dependent DNA polymerase. cDNA was cut by restriction endonucleases and the fragments were terminally labeled by means of polynucleotide kinase and [gamma-32P]ATP. After purification, fragments were degraded by snake venom phosphodiesterase. Alternatively single-stranded [32P]cDNA was prepared by transcription in the presence of [alpha-32P]dCTP and actinomycin D; the product was digested by endonuclease IV and degraded by snake venom phosphodiesterase. cDNA tracts obtained by both labeling methods enabled us to construct a sequence for the translated and 3'-terminal untranslated regions of human beta-mRNA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anemia, Sickle Cell
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA*
  • Dactinomycin
  • Deoxyribonucleases
  • Endonucleases
  • Globins / biosynthesis*
  • Humans
  • Oligodeoxyribonucleotides
  • Phosphoric Diester Hydrolases
  • Polynucleotide 5'-Hydroxyl-Kinase
  • Protein Biosynthesis
  • RNA, Messenger* / metabolism
  • RNA-Directed DNA Polymerase
  • Thalassemia
  • Transcription, Genetic

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Dactinomycin
  • Globins
  • DNA
  • Polynucleotide 5'-Hydroxyl-Kinase
  • RNA-Directed DNA Polymerase
  • Deoxyribonucleases
  • Endonucleases
  • DNA Restriction Enzymes
  • Phosphoric Diester Hydrolases

Associated data

  • GENBANK/J00093
  • GENBANK/J00094
  • GENBANK/J00096
  • GENBANK/J00158
  • GENBANK/J00159
  • GENBANK/J00160
  • GENBANK/J00161
  • GENBANK/J00162
  • GENBANK/J00163
  • GENBANK/J00164
  • GENBANK/J00165
  • GENBANK/J00166
  • GENBANK/J00167
  • GENBANK/J00168
  • GENBANK/J00169
  • GENBANK/J00170
  • GENBANK/J00171
  • GENBANK/J00172
  • GENBANK/J00173
  • GENBANK/J00174
  • GENBANK/J00175
  • GENBANK/J00177
  • GENBANK/J00178
  • GENBANK/J00179
  • GENBANK/K01239
  • GENBANK/K01890
  • GENBANK/K02544
  • GENBANK/M18047
  • GENBANK/M19067
  • GENBANK/X00423