Abstract
The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Polymerase I / metabolism*
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DNA Polymerase II / metabolism*
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DNA Replication / drug effects
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DNA-Directed DNA Polymerase / metabolism*
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Deoxyguanine Nucleotides / metabolism*
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Deoxyguanine Nucleotides / pharmacology
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Thymidine Monophosphate / metabolism*
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Thymine Nucleotides / metabolism*
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Thymine Nucleotides / pharmacology
Substances
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Deoxyguanine Nucleotides
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Thymine Nucleotides
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Thymidine Monophosphate
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O(6)-methyldeoxyguanylic acid
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O(6)-methyldeoxyguanosine triphosphate
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O(4)-methylthymidine monophosphate
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O(4)-methylthymidine triphosphate
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DNA Polymerase I
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DNA Polymerase II
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DNA-Directed DNA Polymerase