Abstract
Four distinct DNA polymerase activities were isolated from ovaries of the frog Xenopus laevis. Specific assays for each activity were established. The isolated activities were characterized by molecular weight, template-primer preferences, and sensitivity to specific inhibitors as Xenopus laevis ovarian DNA polymerases-alpha 1, -alpha 2, -beta, and -gamma. All previously described Xenopus laevis DNA polymerases were classified using these properties.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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DNA Polymerase I / isolation & purification
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DNA Polymerase I / metabolism
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DNA Polymerase II / isolation & purification
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DNA Polymerase II / metabolism
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DNA Polymerase III / isolation & purification
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DNA Polymerase III / metabolism
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DNA-Directed DNA Polymerase / isolation & purification*
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Female
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Kinetics
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Ovary / enzymology*
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Xenopus
Substances
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DNA Polymerase I
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DNA Polymerase II
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DNA Polymerase III
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DNA-Directed DNA Polymerase