Studies on the inhibition of highly purified calf thymus 8S and 7.3S DNA polymerase alpha by aphidicolin

Nucleic Acids Res. 1981 Jan 10;9(1):161-8. doi: 10.1093/nar/9.1.161.

Abstract

On activated DNA aphidicolin competitively inhibits the incorporation of dCMP by both calf thymus DNA polymerase alpha A2 and C enzymes and inhibits the incorporation of the other three deoxynucleoside monophosphates apparently non-competitively. However, aphidicolin does not inhibit the incorporation of dAMP into poly(dT) . oligo(A)10 nor does it inhibit the incorporation of dGMP into poly(dC) . oligo(dG)10, but, it does competitively inhibit the incorporation of dTMP into poly(dA) . oligo(dT)10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin
  • Carbon Radioisotopes
  • Cattle
  • DNA
  • DNA Polymerase II / antagonists & inhibitors*
  • Deoxyribonucleotides
  • Diterpenes / pharmacology*
  • Kinetics
  • Molecular Weight
  • Nucleic Acid Synthesis Inhibitors*
  • Radioisotope Dilution Technique
  • Thymus Gland / enzymology*
  • Tritium

Substances

  • Carbon Radioisotopes
  • Deoxyribonucleotides
  • Diterpenes
  • Nucleic Acid Synthesis Inhibitors
  • Tritium
  • Aphidicolin
  • DNA
  • DNA Polymerase II