Pseudogene IFN-alpha L: removal of the stop codon in the signal sequence permits expression of active human interferon

Gene. 1984 Dec;32(1-2):135-40. doi: 10.1016/0378-1119(84)90041-6.

Abstract

Biologically active interferon (10(6)-10(7) units/liter) was produced in Escherichia coli from modified human alpha interferon (IFN-alpha) pseudogene L. IFN-alpha pseudogene L has a stop codon in the signal peptide coding region. The region that contains the stop codon was replaced with the corresponding region of another human IFN-alpha gene, WA, that does not have a stop codon and was previously engineered for expression by fusion to the M13mp11 lac promoter. The interferon L fusion product was induced with IPTG after infecting E. coli JM103 with the M13 bacteriophage that contained the modified human IFN-alpha pseudogene L. Hence, the IFN-alpha L mature interferon coding sequence, which is not identical to any other alpha-interferon gene, has been conserved for active interferon coding information.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Codon
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression Regulation*
  • Genes
  • Humans
  • Interferon Type I / biosynthesis
  • Interferon Type I / genetics*

Substances

  • Codon
  • Interferon Type I

Associated data

  • GENBANK/M10200
  • GENBANK/M10201