Selective inactivation of the 3' to 5' exonuclease activity of Escherichia coli DNA polymerase I by heat

Nucleic Acids Res. 1983 Nov 11;11(21):7505-15. doi: 10.1093/nar/11.21.7505.

Abstract

Heat selectively inactivates the 3' to 5' exonuclease activity of E. coli DNA polymerase I, resulting in reduced dNTP turnover and lower fidelity of replication of homopolymer and natural DNA templates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Polymerase I / antagonists & inhibitors*
  • DNA Replication
  • Escherichia coli / enzymology*
  • Exodeoxyribonuclease V
  • Exodeoxyribonucleases / antagonists & inhibitors*
  • Hot Temperature
  • Kinetics
  • Templates, Genetic

Substances

  • DNA Polymerase I
  • Exodeoxyribonucleases
  • Exodeoxyribonuclease V