A cellular factor involved in the formation of a DNA-synthesizing complex from DNA polymerase I in Escherichia coli

Biochim Biophys Acta. 1983 Apr 15;739(3):265-75. doi: 10.1016/0167-4781(83)90100-8.

Abstract

A factor ('E') has been identified which stabilizes an endogenous DNA-synthesizing complex involving DNA polymerase I. The complex is separated from free DNA polymerase by polyacrylamide gel electrophoresis. The factor will reform the complex after it has been dissociated and will convert a preparation of DNA polymerase I to complex. The factor and the DNA-synthesizing complex both appear to be localized at the cell membrane.

MeSH terms

  • DNA Polymerase I / isolation & purification
  • DNA Polymerase I / metabolism*
  • DNA Replication*
  • DNA-Directed DNA Polymerase / metabolism*
  • Escherichia coli / enzymology*
  • Kinetics

Substances

  • DNA Polymerase I
  • DNA-Directed DNA Polymerase