Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma

Proc Natl Acad Sci U S A. 1984 Feb;81(3):829-33. doi: 10.1073/pnas.81.3.829.

Abstract

The structures of the rearranged c-myc gene products derived from a rcp T(12;15) have been investigated in the MPC-11 (IgG2b kappa) plasmacytoma. The rcp T(12;15) in MPC-11 is a reciprocal exchange between the c-myc gene on chromosome 15 and the immunoglobulin gamma 2a switch region (S gamma 2a) on chromosome 12. The c-myc gene is broken within a 5'-nontranslated exon, thereby separating the promoter region of the normal c-myc gene from its protein coding sequences. This reciprocal rearrangement results in the loss of 11 base pairs of c-myc sequence and 300 base pairs of S gamma 2a sequence at the point of recombination. Sequences that represent the promoter region of the normal c-myc gene are present in the 5'-myc reciprocal fragment. A comparison of the nucleotide sequences at the recombination site of a number of c-myc rearrangements reveals a common feature that may have mechanistic importance for these translocation events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Endonucleases
  • Mice
  • Oncogenes*
  • Plasmacytoma / genetics*
  • Single-Strand Specific DNA and RNA Endonucleases
  • Translocation, Genetic*

Substances

  • Endonucleases
  • DNA Restriction Enzymes
  • Single-Strand Specific DNA and RNA Endonucleases

Associated data

  • GENBANK/J00373
  • GENBANK/J00374
  • GENBANK/K01441
  • GENBANK/K01442
  • GENBANK/K01865
  • GENBANK/K01866
  • GENBANK/L00038