A helix-destabilising protein from herpes simplex virus type I infected cells which specifically stimulates the virus induced DNA polymerase activity in vitro

Biochem Biophys Res Commun. 1983 Oct 14;116(1):327-34. doi: 10.1016/0006-291x(83)90418-7.

Abstract

We have isolated a protein fraction from HSV-1 infected cells which binds specifically to single-stranded DNA, facilitates a lowering of the melting temperature of poly[d(A-T)] and specifically stimulates the activity of the homologous virus-induced DNA-dependent DNA polymerase in vitro. These are major characteristics of a helix-destabilising protein, exemplified by the prokaryotic gene 32 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / physiology*
  • DNA-Directed DNA Polymerase / metabolism*
  • Enzyme Activation
  • Poly dA-dT / genetics
  • Simplexvirus / genetics*
  • Virus Replication*

Substances

  • DNA-Binding Proteins
  • Poly dA-dT
  • DNA-Directed DNA Polymerase