Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

Nature. 1982 Jan 21;295(5846):202-6. doi: 10.1038/295202a0.

Abstract

The nucleotide sequence of cloned cDNA for preproenkephalin from bovine adrenal medulla indicates that the precursor protein contains four copies of Met-enkephalin and one copy each of Leu-enkephalin, Met-enkephalin-Arg6-Phe7 and Met-enkephalin-Arg6-Gly7-Leu8, a previously undetected opioid peptide. The enkephalin and extended enkephalin sequences are each bounded by paired basic amino acid residues. Preproenkephalin may represent a multi-hormone precursor, like the corticotropin-beta-lipotropin precursor.

MeSH terms

  • Adrenal Glands / metabolism*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA*
  • Endorphins / genetics*
  • Enkephalins / genetics*
  • Protein Biosynthesis
  • Protein Precursors / genetics*
  • RNA, Messenger / genetics
  • Transcription, Genetic

Substances

  • Endorphins
  • Enkephalins
  • Protein Precursors
  • RNA, Messenger
  • DNA
  • preproenkephalin
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J00005