Abstract
The 3' à 5' exonuclease activity of E. coli DNA-polymerase I is inhibited by nucleotides and deoxynucleotides at concentrations (< 1 mM) where polymerase activity is not affected. This inhibitory effect depends on the nature of the excised deoxynucleotide, excision of purines being much less inhibited than that of pyrimidines. It does not depend on the purine or pyrimidine nature of the inhibitor.
MeSH terms
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Bacterial Proteins / metabolism
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DNA Polymerase I / metabolism*
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DNA-Directed DNA Polymerase / metabolism*
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Deoxyribonucleotides / analysis
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Deoxyribonucleotides / pharmacology*
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Escherichia coli / enzymology*
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Exonucleases / antagonists & inhibitors*
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Purine Nucleotides / analysis
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Purine Nucleotides / pharmacology*
Substances
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Bacterial Proteins
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Deoxyribonucleotides
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Purine Nucleotides
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DNA Polymerase I
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DNA-Directed DNA Polymerase
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Exonucleases