Steady-state enzyme kinetics of the pancreatic ribonucleases from five mannalian species

Biochim Biophys Acta. 1976 May 13;429(3):853-9. doi: 10.1016/0005-2744(76)90331-4.

Abstract

The kinetic parameters Km, k+2 and k+2/Km of the pancreatic ribonucleases (EC 3.1.4.22) from cow, giraffe, horse, rat and lesser rorqual have been determined, using 2',3'-cyclic cytidine monophosphate and 2',3'-cuclic uridine monophosphate as substrates. No large differences were found between the activities of the five enzymes. The relative differences between the activities of the five enzymes are mainly due to differences in the rates of hydrolysis and not to differences in the affinities for the substrates.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Artiodactyla
  • Cattle
  • Cytosine Nucleotides / metabolism
  • Female
  • Horses
  • Hydrogen-Ion Concentration
  • Kinetics
  • Nucleotides, Cyclic / metabolism
  • Pancreas / enzymology*
  • Rats
  • Ribonucleases / metabolism*
  • Uracil Nucleotides / metabolism
  • Whales

Substances

  • Cytosine Nucleotides
  • Nucleotides, Cyclic
  • Uracil Nucleotides
  • Ribonucleases