Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli

Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.

Abstract

The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.

MeSH terms

  • Adenine Nucleotides
  • Adenosine Triphosphate / metabolism*
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Chromatography, DEAE-Cellulose
  • Chromatography, Ion Exchange
  • Coliphages*
  • DNA Nucleotidyltransferases / metabolism*
  • DNA, Single-Stranded / metabolism
  • DNA, Viral / biosynthesis*
  • DNA, Viral / isolation & purification
  • Escherichia coli / analysis
  • Escherichia coli / enzymology*
  • In Vitro Techniques
  • Nucleic Acid Hybridization
  • Nucleotides / metabolism
  • Phosphorus Radioisotopes
  • Polynucleotides
  • RNA, Viral
  • Templates, Genetic
  • Thymine Nucleotides / metabolism
  • Tritium

Substances

  • Adenine Nucleotides
  • Bacterial Proteins
  • DNA, Single-Stranded
  • DNA, Viral
  • Nucleotides
  • Phosphorus Radioisotopes
  • Polynucleotides
  • RNA, Viral
  • Thymine Nucleotides
  • Tritium
  • Adenosine Triphosphate
  • DNA Nucleotidyltransferases