Abstract
The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
MeSH terms
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Adenine Nucleotides
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Adenosine Triphosphate / metabolism*
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism*
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Chromatography, DEAE-Cellulose
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Chromatography, Ion Exchange
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Coliphages*
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DNA Nucleotidyltransferases / metabolism*
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DNA, Single-Stranded / metabolism
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DNA, Viral / biosynthesis*
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DNA, Viral / isolation & purification
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Escherichia coli / analysis
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Escherichia coli / enzymology*
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In Vitro Techniques
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Nucleic Acid Hybridization
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Nucleotides / metabolism
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Phosphorus Radioisotopes
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Polynucleotides
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RNA, Viral
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Templates, Genetic
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Thymine Nucleotides / metabolism
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Tritium
Substances
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Adenine Nucleotides
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Bacterial Proteins
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DNA, Single-Stranded
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DNA, Viral
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Nucleotides
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Phosphorus Radioisotopes
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Polynucleotides
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RNA, Viral
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Thymine Nucleotides
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Tritium
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Adenosine Triphosphate
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DNA Nucleotidyltransferases