Abstract
Evidence is presented that DNA polymerases from human leukemic cells are mutagenic. Nucleic acid-free extracts of acute lymphoblastic leukemic cells polymerized about 10-times more dCTP using poly(dA-dT).poly(dA-dT) as a template than did extracts from normal lymphocytes. Mutagenic DNA polymerases could perhaps play an important role in tumor progression.
MeSH terms
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Adenosine Triphosphate / metabolism
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Centrifugation, Density Gradient
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Cytosine Nucleotides
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DNA Nucleotidyltransferases / blood*
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DNA Replication
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DNA, Neoplasm / analysis
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DNA, Neoplasm / biosynthesis
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DNA, Neoplasm / metabolism
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Guanosine Triphosphate / metabolism
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Humans
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Leukemia, Lymphoid / enzymology*
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Lymphocytes / enzymology
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Mutation*
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Phosphoric Diester Hydrolases / metabolism
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Phosphorus Isotopes
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Polynucleotides / metabolism
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Templates, Genetic
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Thymine Nucleotides / metabolism
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Tritium
Substances
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Cytosine Nucleotides
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DNA, Neoplasm
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Phosphorus Isotopes
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Polynucleotides
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Thymine Nucleotides
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Tritium
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Guanosine Triphosphate
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Adenosine Triphosphate
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DNA Nucleotidyltransferases
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Phosphoric Diester Hydrolases