Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro

Y Ohashi; A Itaya; Y Tanaka; K Yoshihara; T Kamiya; A Matsukage

doi:10.1016/0006-291x(86)90783-7

Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro

Biochem Biophys Res Commun. 1986 Oct 30;140(2):666-73. doi: 10.1016/0006-291x(86)90783-7.

Authors

Y Ohashi, A Itaya, Y Tanaka, K Yoshihara, T Kamiya, A Matsukage

PMID: 3778475
DOI: 10.1016/0006-291x(86)90783-7

Abstract

DNA polymerase beta purified from bovine thymus is markedly inhibited when incubated in a reconstituted poly(ADP-ribosyl)ating reaction system. Analyses of the reaction product synthesized in this system by SDS-polyacrylamide gel electrophoresis and subsequent fluorography of the gel indicated that ADP-ribose is covalently attached to DNA polymerase beta molecule (Mr = 44,000).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Polymerase I / metabolism*
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Nucleoside Diphosphate Sugars / metabolism*
Photofluorography
Poly Adenosine Diphosphate Ribose / metabolism*
Protein Binding

Substances

Nucleoside Diphosphate Sugars
Poly Adenosine Diphosphate Ribose
DNA Polymerase I